Coding

Part:BBa_K3256440:Design

Designed by: CHEN YU-SYUN   Group: iGEM19_NCTU_Formosa   (2019-10-20)


ccdB*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Be sure that there is no any normal restriction site in the sequence


Source

https://www.uniprot.org/uniprot/P62554

References

Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. Positive-selection vectors using the F plasmid ccdB killer gene." Gene 1994 Oct 11;148(1):71-4. pmid:7926841